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It looks like you have two Aliases for the same path. So hence you have a duplication. Remove:
Alias /plagtest/ "/Users/plagtest/myproject/"
<Directory "/Users/plagtest/myproject/">
Order allow,deny
Options Indexes
Allow from all
IndexOptions FancyIndexing
</Directory>
Without the media alias’s the django manuals recommended HTTP conf is:
WSGIScriptAlias / /path/to/mysite.com/mysite/wsgi.py
<Directory /path/to/mysite.com/mysite>
<Files wsgi.py>
Order allow,deny
Allow from all
</Files>
</Directory>
Source:stackexchange.com